Paper Microfluidic Extraction of Bacterial and Viral Nucleic Acid from Field and Clinical Samples towards a Direct Microtas Apparatus

We investigated a rapid, paper microfluidic-based protocol for extracting nucleic acid from a variety of pathogenic samples and developed a multi-faceted paper channel to test for optimal extraction conditions. Salmonella Typhimuirum and Influenza A/H1N1 (seasonal) samples were loaded on the paper strips and were lysed. TE buffer was loaded for elution, and three sections of paper at specified lengths were excised for elution. The limit of detection of S. Typhimuirum in 10% poultry packaging liquid extracted in cellulose paper was 10 3 CFU/mL, which was significantly lower than that of 10 6 CFU/mL when extracted in nitrocellulose paper. We observed that DNA migrates through nitrocellulose at a higher rate and further than through cellulose, and larger genomic DNA separates from smaller plasmid DNA in both paper types.